Figure 4.

Renal fibrosis in each group (n = 3–8)

(A) Periodic acid-Schiff (PAS) staining images of renal tissue at the age of 12,18 and 21 months in each group.

(B) Graph represents the area score of renal interstitial matrix after PAS staining. Scale bars: 100mm.

(C) The trends of renal fibrosis showed by PAS staining with aging.

(D) Sirius red staining images of renal tissue at the age of 12, 18, and 21 months in each group.

(E) Graph represents area score of renal fibrosis after Sirius red staining.

(F) The trends of renal fibrosis showed by Sirius red staining with aging. Data shown as mean ± SD. ▲ p＜0.05, ▲▲ p < 0.01, ▲▲▲ p < 0.001, ▲▲▲▲p＜0.0001 versus the control group, ∗∗∗∗p < 0.0001 versus 1/6NX group, ##p＜0.01, ####p＜0.0001 versus the UNX group and Sirius red staining of rats in each group.