REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal to P16	Abcam	ab51243; RRID: AB_2059963
Mouse monoclonal to P21	Santa Cruz Biotechnology	Cat# sc-6246; RRID: AB_628073
Mouse monoclonal to P53	Cell Signaling Technology	Cat# 2524; RRID: AB_331743
Rabbit polyclonal to γH2AX	Cell Signaling Technology	Cat# 2577; RRID: AB_2118010
Mouse monoclonal to IL-6	Santa Cruz Biotechnology	Cat# sc-57315; RRID: AB_2127596
Rabbit monoclonal to p-nuclear factor κB	Cell Signaling Technology	Cat# 3033; RRID: AB_331284
Rabbit polyclonal to TNF-α	Proteintech	Cat# 17590-1-AP; RRID: AB_2271853
Rabbit monoclonal to G6PD	Abcam	Cat# ab133525; RRID:AB_2904024
Rabbit monoclonal to COL-Ⅰ	Abcam	Cat# ab138492; RRID: AB_2861258
Rabbit monoclonal to COL-Ⅲ	Abcam	Cat# ab184993; RRID: AB_2895112
Chemicals, peptides, and recombinant proteins
Recombinant Human TNF-α	Solarbio	P00029
Recombinant Human IL-6	Solarbio	P00022
Critical commercial assays
Rat SA-β-gal elisa kit	Jianglaibio	JL54746
Senescence β-Galactosidase Staining Kit	Beyotime Biotechnology	C0602
Glucose 6 phosphate dehydrogenase assay kit	Solarbio	BC0260
NADP/NADPH assay kit	Solarbio	BC 1105
Reactive Oxygen Species Assay Kit	Beyotime Biotechnology	S0033S
Tissue reactive oxygen species detection kit	BIO-LAB	HR8820
Dihydroethidium	Beyotime Biotechnology	S0063
Total Superoxide Dismutase Assay Kit	Beyotime Biotechnology	S0101S
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)	Calibra Scientific, Inc.	N/A
Deposited data
metabolomics data	N/A	https://www.ebi.ac.uk/metabolights/MTBLS10106
Experimental models: Cell lines
HK-2 cell	ATCC	CRL-2190
Experimental models: Organisms/strains
Wistar rats	Experimental Animal Center of General Hospital of PLA	N/A
Oligonucleotides
siRNA targeting sequence: G6PD see Table S1	Cyagen Biosciences Inc	N/A
qRT-PCR primers, see Table S2	Ji Ma Gene Company	N/A
Software and algorithms
ImageJ	National Institute of health	https://imagej.nih.gov/ij/
GraphPad Prism software	GraphPad	https://www.graphpad.com