FIG. 7.

Recovery of HIV-1 fusion activity by reconstitution of PPMP-treated cells with GSLs. Lipids were incorporated into liposomes and transferred to control and PPMP-treated GHOST-X4 and GHOST-R5 cells as described in Materials and Methods. Fusion activity was monitored using vaccinia virus vectors which express gp120-gp41 from an X4-utilizing isolate (IIIB, vpE16), an R5-utilizing isolate (Ba-L, vcB43), and an X4R5-utilizing isolate (89.6, vDC-1) in HeLa cells. (A) GHOST-X4 cells as targets; (B) GHOST-R5 cells as targets. PPMP (−) GHOST-X4 and GHOST-R5 are the untreated cells; PPMP (+) GHOST-X4 and GHOST-R5 are the PPMP-treated cells without addition of GSL; PPMP (+) GHOST-X4 and GHOST-R5 + GSL are the PPMP-treated cells with addition of the indicated GSL.