Figure 5.

ASCC1 overlaid with structural orthologs highlights conserved and unique ASCC1 structural elements.A, ApASCC1 KH domain overlaid onto structural orthologs complexed to RNA, suggests which critical motifs are conserved and which are inconsistent with current ASCC1 structure. KH domains were superimposed over whole ASCC1 KH domain. Additional overlays are in Supporting information. B, ApASCC1 PDE domain overlaid onto structural orthologs complexed to RNA or ligand suggests which critical motifs are conserved and which are inconsistent with current ASCC1 structure. PDE domains were superimposed based on the two HXT motifs (labeled as 1 and 2 in figure). Additional overlays are in Supporting information. C, ApASCC1 KH and PDE domain with superimposed substrate based upon superimposition of protein domains as in A and B. D, Superimposition of base 1 (∗, base numbering based on Fig. 6) from KH-bound RNA onto PDE-bound RNA, relative to ApASCC1 HXT motifs. Sugar puckers were both C2′ endo.