Figure 2.

GSK reverses the effects of HS on Th17 and Treg program in vitro

(A) Schematic representation of Th17 and Treg in vitro differentiation.

(B and C) The proportion of differentiated Th17 cells (n = 3).

(D and E) The proportion of differentiated Treg cells (n = 3).

(F and G) Naive CD4 T cells were cultured for 3 days under the Treg polarization condition. Then, Treg cells were harvested, washed and cultured for an additional 2 days under Th17 condition in the presence of HS and/or GSK. The proportion of IL-17A producing cells within the Foxp3⁺ Treg cells was shown (n = 3). Statistical significance was calculated by unpaired Student’s t test and data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.