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Translation of the Flag ORF in A20 cells. A20 cells were transfected with the indicated plasmids. After 24 h, cells were lysed with NP-40 and the cell lysate was analyzed by Western blotting using MAb M2, which is specific for the Flag epitope. A predicted 18-kDa band is visible in the case of the flagged ORF (pHKputSag1-FLAG). pCIneo-DMSag and pHKputSag1 served as negative controls. The experiment was repeated with identical results.
