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. 2024 Jun 28;15:5497. doi: 10.1038/s41467-024-49879-6

Fig. 2. Re-infection triggers an early transcriptional response from Th1-memory PbTIIs.

Fig. 2

A Schematic of scRNA-seq experiment to study in vivo responses of antigen-experienced versus naïve PbTII cells during re-infection. B–D Representative FACS plots showing B IFN-γ, C CXCR6, and D Ki67 expression in antigen-experienced PbTII cells at day 28 p.i. and 1 and 3 days post re-infection, compared to co-transferred naïve comparator PbTIIs. Data combined from two independent experiments showing similar results (n = 9 mice for day 28 p.i, 15 mice each for 1 and 3 days post-re-infection); two-way ANOVA with Šídák’s multiple comparison testing; ****P < 0.0001. E UMAP of antigen-experienced PbTII cells and naïve comparators, prior to and 1 day after re-infection. F UMAPs of PbTII expression of various genes associated with Th1 (Cxcr6, Ifng), Tfh (Cxcr5, Bcl6), TCM (Ccr7, Sell, Tcf7, Klf2), proliferation (Mki67), Sostdc1+ cells, and early activation (Cd69).