Fig. 8. Th1-memory clones exhibit a robust proliferative Tr1 recall response during re-infection.

A UCSC genome browser tracks displaying genomic accessibility signals around the Il10 gene locus. (Top) Mean bulk ATAC-seq genome coverage. Boxes at the top of each coverage represent peaks called using MACS2. Data representative of two independent experiments showing similar results²³. (Middle) Accessibility profiles of 200 randomly selected single cells from plate-based single-cell ATAC-seq data²³. (Bottom) Percentage of cells with open accessibility at each genomic region B UMAP of PbTII cells and polyclonal CD4⁺ T cells showing mRNA expression of Ifng, Il10, Il2, Tnf, Ctla4, Lag3, Havcr2, and Tigit. C UMAP of PbTII cells (Left) and polyclonal CD4⁺ T cells (Right) with Il10 and Ifng co-expressing cells highlighted in red. D UMAP of CD11a^hiCXCR3⁺ polyclonal CD4⁺ T cells (with 5% spike-in of CD11a^loCXCR3⁻ naïve cells) showing TCR sharing (straight edges) from those cells co-expressing Il10 and Ifng (highlighted in open circles and coloured by mouse of origin; n = 5 mice).