Primary Antibody | Company | Dilution 5% [w/v] | Source | Incubation | Secondary Antibody (LI-COR) |
---|---|---|---|---|---|
α-HA.11 | Covance (clone 16B12) | 1:1000 / milk | mouse, mAb | o/n // 4°C | IRDye 800CW Goat α-mouse IgG |
α- β Actin | Abcam (ab6276) | 1:10000 / milk | mouse, mAb | o/n // 4°C | IRDye 800CW Goat α-mouse IgG |
α-METTL8 | Helmholtz Center Munich (MEL8 16A7; 19A10) | 1:500 / milk | rat, mAb | o/n // 4°C | IRDye 800CW Goat α-rat IgG |
α-TIMM44 | ProteinTech | 1:3000 / milk | rabbit, pAb | o/n // 4°C | IRDye 800CW Goat α-rabbit IgG |
α-GAPDH | GeneTEx (GT239) | 1:1000 / milk | mouse, mAb | o/n // 4°C | IRDye 800CW Goat α-mouse IgG |
α-p54[nrb] | BD Transduction Laboratories | 1:1000 / milk | mouse, mAb | o/n // 4°C | IRDye 800CW Goat α-mouse IgG |