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. Author manuscript; available in PMC: 2024 Jun 30.
Published in final edited form as: Mol Cell. 2021 Nov 12;81(23):4810–4825.e12. doi: 10.1016/j.molcel.2021.10.018
Primary Antibody Company Dilution 5% [w/v] Source Incubation Secondary Antibody (LI-COR)
α-HA.11 Covance (clone 16B12) 1:1000 / milk mouse, mAb o/n // 4°C IRDye 800CW Goat α-mouse IgG
α- β Actin Abcam (ab6276) 1:10000 / milk mouse, mAb o/n // 4°C IRDye 800CW Goat α-mouse IgG
α-METTL8 Helmholtz Center Munich (MEL8 16A7; 19A10) 1:500 / milk rat, mAb o/n // 4°C IRDye 800CW Goat α-rat IgG
α-TIMM44 ProteinTech 1:3000 / milk rabbit, pAb o/n // 4°C IRDye 800CW Goat α-rabbit IgG
α-GAPDH GeneTEx (GT239) 1:1000 / milk mouse, mAb o/n // 4°C IRDye 800CW Goat α-mouse IgG
α-p54[nrb] BD Transduction Laboratories 1:1000 / milk mouse, mAb o/n // 4°C IRDye 800CW Goat α-mouse IgG