Fig. 1.

Genetic disruption of ATAT1 inhibits stress fiber and focal adhesion formation and reduces RhoA expression and activity. (A) Immunocytochemistry analysis of acetylated α-tubulin, focal adhesions and F-actin in mock and ATAT1 KO cells. Scale bar, 10 μm. Graphs show the lengths of actin stress fibers between two focal adhesions and area of focal adhesions at the marginal region (n > 100). (B) Functional annotation of 3,644 genes downregulated in ATAT1 KO cells using PANTHER and GO database and schematic diagram of the identification of the RHOA gene. (C) Results from qPCR analysis of the transcriptional expression of RHOA in mock and ATAT1 KO (clones #1 and #2) MDA-MB-231 cells. (D) Immunoblotting analysis showing the protein level of RhoA in mock and ATAT1 KO cells. (E) A RhoA activity assay was performed on mock and ATAT1 KO cells. All error bars represent the standard deviation (SD) of the data.