FIG. 3.
Changes in SM levels and effect of fumonisin B1 on ceramide generation in response to NSV infection. (A) SM degradation in N18 cells labeled with [3H]choline and then infected with NSV (MOI = 50). Lipids were extracted, and SM was resolved by thin-layer chromatography and quantified by liquid scintillation counting. The values represent means ± standard deviations (SD) (error bars) of the SM levels in NSV-infected cells as a percentage of mock-infected cells × 100. (B) N18 cells treated with 100 μM fumonisin B1 (FB1) and then mock infected or infected with NSV (MOI = 50). At 6 and 12 h after infection, cellular lipids were extracted and assayed for ceramide as described in the legend to Fig. 1. Values represent means ± SD (error bars).