Table 2.
Recommended antibodies for assessment of LysoIP efficiency and purity
| Target | Source and catalog number | Concentration | Approximative running size |
|---|---|---|---|
| HA (Tag) | Cell Signaling Technology (Cat#3724) | 1:10,000 | 37 kDa |
| LAMP2 (Lysosomes) | Santa Cruz Biotechnology (Cat#sc-18822) | 1:5,000 | 100 kDa |
| CTSD (Lysosomes) | BD Biosciences (Cat#610800) | 1:1,000 | 50–25 kDa |
| VDAC1 (Mitochondria) | Cell Signaling Technology (Cat#4661) | 1:1,000 | 37 kDa |
| CALRETICULIN (Endoplasmic Reticulum) | Cell Signaling Technology (Cat#12238) | 1:1,000 | 50 kDa |
| GM130 (Golgi Apparatus) | Abcam (Cat#ab52649) | 1:1,000 | 130 kDa |
| EEA1 (Early Endosomes) | BD Biosciences (Cat#610456) | 1:1,000 | 150 kDa |