| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CALRETICULIN 1:1,000 | Cell Signaling Technology | Cat#12238; RRID:AB_2688013 |
| CATHEPSIN D 1:1,000 | BD Biosciences | Cat#610800; RRID:AB_398119 |
| EEA1 1:1,000 | BD Biosciences | Cat#610456; RRID:AB_397829 |
| FLAG 1:1,000 | Cell Signaling Technology | Cat#F1804; RRID:AB_262044 |
| GM130 1:1,000 | Abcam | Cat#ab52649; RRID:AB_880266 |
| HA 1:10,000 | Cell Signaling Technology | Cat#3724; RRID:AB_1549585 |
| LAMP2 1:5,000 | Santa Cruz Biotechnology | Cat#sc-18822; RRID:AB_626858 |
| VDAC 1:1,000 | Cell Signaling Technology | Cat#4661; RRID:AB_10557420 |
| Goat anti-mouse IgG1, human ads-HRP 1:5,000 | SouthernBiotech | Cat#1070-05 |
| Goat anti-rabbit IgG (H + L), mouse/human ads-HRP 1:5,000 | SouthernBiotech | Cat#4050-05 |
| Goat anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor 546 1:500 | Life Technologies | Cat#A-11035 |
| Goat anti-mouse IgG1 cross-adsorbed secondary antibody, Alexa Fluor 488 1:500 | Life Technologies | Cat#A-21121 |
| Biological samples | ||
| Patient-derived glioblastoma stem-like cells GSC#9 | Harford-Wright E. et al. (2017) | N/A |
| Experimental models: Cell lines | ||
| Human embryonic kidney cells HEK293T | ATCC | Cat#CRL-3216; RRID:CVCL_0063 |
| Chemicals, peptides, and recombinant proteins | ||
| Tris-HCl | Millipore | Cat#648313 |
| Chloroquine | Sigma | Cat#C6628 |
| NaCl | VWR | Cat#27810.295 |
| Polybrene | Santa Cruz Biotechnology | Cat#sc-134220 |
| EDTA | Millipore | Cat#324504 |
| Triton X-100 | Sigma | Cat#T9284 |
| IGEPAL | Sigma | Cat#I8896 |
| SDS 20% | Euromedex | Cat#EU0660-B |
| HEPES | Life Technologies | Cat#12104123 |
| NaH2PO4 | Sigma | Cat#71507 |
| CaCl2 | Sigma | Cat#21115 |
| 2-mercaptoethanol | Carl Roth | Cat#4227 |
| DAPI solution | Life Technologies | Cat#62248 |
| Recombinant DNA | ||
| pLJC5-Tmem192-3×HA | Addgene | Cat#102930; RRID:Addgene_102930 |
| pLJC5-Tmem192-2×FLAG | Addgene | Cat#102929; RRID:Addgene_102929 |
| psPAX2 | Addgene | Cat#12260 |
| pCMV-VSV-G | Addgene | Cat#8454 |
| Other | ||
| N2 supplement | Life Technologies | Cat#17502048 |
| G5 supplement | Life Technologies | Cat# 17503012 |
| B27 supplement | Life Technologies | Cat#17504044 |
| 100× Halt protease inhibitor cocktail | Life Technologies | Cat#78429 |
| Pierce anti-HA magnetic beads | Life Technologies | Cat#88837 |
| DMEM-F12 | Life Technologies | Cat#10565018 |
| DMEM | Life Technologies | Cat#11995 |
| 0.05% Trypsin-EDTA | Life Technologies | Cat#2500054 |
| Penicillin/streptomycin solution | Life Technologies | Cat#15140130 |
| Trypan blue stain 0.4% | NanoEntek | Cat#20230411 |
| Amphotericin B | Life Technologies | Cat#15290026 |
| Puromycin | Life Technologies | Cat#J67236.8EQ |
| GlutaMAX | Life Technologies | Cat#35050061 |
| Magnet DynagMag-2 | Thermo Fisher Scientific | Cat#12321D |
| SDS sample buffer | Life Technologies | Cat#LC2676 |
| Fetal bovine serum (FBS) | Corning | Cat#35079CV |
| Paraformaldehyde | Electron Microscopy Sciences | Cat#15710; CAS: 30525-89-4 |
| Triton X-100 | Merck Millipore | Cat#T9284; CAS: 9036-19-5 |
| Sterile 0.5/1 mL insulin syringe with 12.7 mm 29-gauge needle (33/100) | BD Micro-Fine | Cat#324892 |
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