NS34 culture media

Reagent	Final concentration	Amount
Glutamax (100×)	200 μM	5 mL
Penicillin/streptomycin (100×)	100 units each	5 mL
Amphotericin B (250 μg/mL)	0.25 μg/mL	500 μL
N2 supplement (100×)	1×	5 mL
G5 supplement (100×)	0.2×	1 mL
B27 supplement (50×)	1×	10 mL
DMEM-F12	N/A	qsf 500 mL
Total	N/A	500 mL

Note: NS34 culture media can be stored at 4°C for several weeks.