FIG. 2.
Heparin-agarose affinity chromatography of RSV-F proteins. Purified RSV proteins or infected cell lysates from recombinant vaccinia viruses expressing either RSV-G (Ga, subgroup A; Gb, subgroup B) or RSV-F were subject to HAAC followed by Western blot analysis using a polyclonal rabbit anti-G (A) or anti-F (B) antiserum. The assay was carried out as described in Materials and Methods except that purified proteins were used at a concentration of 50 μg/ml of heparin-agarose. (A) Purified RSV-Ga (lane 1), vaccinia virus-expressed RSV-Ga (lane 2), and vaccinia virus-expressed RSV-Gb (lane 3). (B) Purified RSV-Fa (lane 1) and vaccinia virus-expressed RSV-Fa (lane 2). G90 represents the mature, fully glycosylated form of RSV-G.