FIG. 3.

Heparin-agarose affinity chromatography of RSV cp-52-infected Vero cell lysate. Heparin chromatography was performed as described in the legend to Fig. 1, followed by Western blot analysis using an anti-F polyclonal antiserum (lane 1, infected cell lysate; lane 2, final wash; lane 3, heparin-eluted material). To demonstrate that cp-52 is unable to synthesize the G and SH proteins, cp-52 lysate was probed with an anti-G polyclonal antiserum (lane 4) or an anti-SH antiserum (lane 6), and lysate from A2-infected cells was included as a control for anti-G (lane 5) and anti-SH (lane 7) antibody reactivity. G₉₀ represents the mature, fully glycosylated form of RSV-G. G₅₅ is a partially glycosylated precursor of G₉₀. SH₀ represents the full-length unglycosylated form of RSV-SH.