FIG. 5.

Effects of GAPDH overexpression (A) or PTB expression (B) on either the wt (bars 1, 3, and 5) or the HM175/P16 (bars 2, 4, and 6) IRES elements in three different cell lines: BSC-1 (bars 1 and 2), FRhK-4 (bars 3 and 4), and Huh-7 (bars 5 and 6). RLuc activities in cells transfected with dicistronic expression constructs containing either the wt or P16 IRES were normalized to those present in cells transfected in parallel with the corresponding fsGAPDH or fsPTB null-expression plasmids. The results shown are means of the values obtained in two independent transfection experiments, each done in triplicate (a total of six transfections). Error bars indicate SD.