Figure 5.

S3372C causes metaphase arrest in the embryo. (A) Example widefield images of fixed embryos from a 2- to 4-h egg collection from mated control (yw) and S3372C mothers stained with DAPI (arrowheads show examples of DNA staining). (B) Quantification of stages of control and S3372C embryos from 2- to 4-h egg collections. N, number of embryos scored. (C) Example confocal images of mitotic spindles in fixed control and S3372C embryos (Z-projection) stained with antibodies to Centrosomin (Cnn; centrosome marker) and α1-tubulin (α1-Tub; microtubules), as well as DAPI. (D) Example stills from time series (single focal plane) of control and S3372C embryos acquired during preblastoderm cycles. Jupiter-GFP and His2Av-mRFP label microtubules and chromatin, respectively. Timestamps are min:s. Scale bars: A, 100 µm; C and D, 20 µm.