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. 2024 Jul 1;223(10):e202310022. doi: 10.1083/jcb.202310022

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© 2024 MRC Laboratory of Molecular Biology

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 6. — S3372C causes ectopic accumulation of dynein in the vicinity of kinetochores. (A) Example confocal images (Z-projections) of mitotic spindles in live control and S3372C (top panels) and S3372C/+ (bottom panels) embryos expressing fluorescently labeled dynein (GFP-Dlic). Embryos additionally contain markers of kinetochores (Spc25-RFP; top) or chromatin (Histone H2A-RFP; bottom). In the bottom panels, the arrow and arrowhead show examples of accumulation of dynein in the vicinity of a subset of chromosomes at the metaphase plate in S3372C/+ embryos. Scale bars: 10 µm. (B) Quantification of GFP-Dlic signal at the equator versus pole regions for the indicated genotypes. Columns show the mean of “per spindle” equator enrichment values; error bars represent SD; circles are values for individual spindles. Number of spindles analyzed shown above bars (number of embryos analyzed shown in parentheses). Statistical significance was evaluated with a one-way ANOVA with Dunnett’s multiple comparisons test, comparing the per spindle values. *, P < 0.05; ****, P < 0.0001.