Video 1.
Live imaging of microtubules and chromatin in embryonic mitosis. Composite of example movies (generated with spinning disk confocal microscopy) of a single focal plane of control and S3372C preblastoderm cycle embryos. Jupiter-GFP (green) and His2Av-mRFP (magenta) label microtubules and chromatin, respectively. In 57 out of 60 movies (of 15-min duration each) of S3372C embryos that expressed fluorescent markers of the mitotic apparatus, no progression of metaphase-arrested spindles was observed. In the remaining movies, progression of highly abnormal mitotic spindles that were formed by fusion of two spindles was seen. Images were collected at 0.2 frames/s with a playback rate of 10 frames/s. Timestamps are min:s. Scale bar, 10 µm. Related to Fig. 5.