FIG. 5.

Vectorial release of HAV from apically infected polarized Caco-2 cell monolayers. Cells were inoculated as in Fig. 2 and washed prior to refeeding following the 2-h adsorption period. Apical and basolateral supernatant fluids were collected at 24-h intervals for virus titration and replaced with fresh media. (A) Virus content of apical (open bars) and basolateral (shaded bars) supernatant fluids. The values shown represent the mean virus titer of fluids from three replicate infected cultures (± standard deviation). (B) Proportion of all released virus (apical plus basolateral supernatant fluid virus) released into apical (open bars) or basolateral (shaded bars) fluids. The results shown represent the means of three replicate cultures at each time point, as in panel A.