FIG. 7.

Brefeldin A (BFA) inhibits apical release of progeny HAV. Cells were treated with various concentrations of brefeldin A beginning immediately after removal of an apical HAV inoculum, as in Fig. 6A. The proportion of the total virus yield (cell-associated plus apical and basolateral fluid virus) that was present in the apical (A) or basolateral (B) supernatant fluids 72 h postinfection was calculated for each culture. The results shown represent the mean (± range) results for replicate cultures treated with the drug and the mean (± standard deviation) of five cultures maintained in the absence of brefeldin A. Samples of apical and basolateral fluids from cells treated with 10 μg of brefeldin A per ml could not be assayed for HAV due to residual antiviral activity.