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. 2024 Jun 3;213(2):214–225. doi: 10.4049/jimmunol.2400007

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FIGURE 2. — Single-cell sequencing analysis of CCR1/2+ve monocytes. (A) Two-dimensional UMAP visualization of bone marrow–derived CCR1/2+ve monocytes. Colors represent individual clusters of cells (0–4). Resolution = 0.21. (B) Heatmap depicting the top 20 differentially expressed genes within the CCR1/2+ve monocyte population that scaled during processing. (C) UMAP visualization of cell cycle analysis. (D) (i–vii) Feature plots depicting gene expression patterns of H2-Ab1, Elane, Ncam1, S100a9, Slpi, Ccr2, and Ccl6, respectively. (E) UMAP visualization of CCR1/2+ve monocytes with the effect of cell cycle regressed out of the data. (F) Heatmap depicting the top 20 genes that scaled during processing for each cluster after cell cycle regression.