TABLE 1.

Oligonucleotides used in this study

Application	Oligonucleotide	Position in satC or diG	Sequencea	Polarityb
Site-directed mutagenesis	C5′	1–19 (satC)	5′ GGGATAACTAAGGGTTTCT 3′	+
	C5C*	329–356 (satC)	5′ GGGCAGGCCCCCCCCCCGCGCGAGGAGGGAGG 3′	−
	C6G	329–356 (satC)	5′ GGGCAGGCCCCCCCCCCGAGGAGGGAGG 3′	−
	C56G	329–356 (satC)	5′ GGGCAGGCCCCCCCCCCGCGAGGAGGGAGG 3′	−
	G5′	1–19 (diG)	5′ GGGATAAAAAGGAGGCTTA 3′	+
	G5C	313–344 (diG)	5′ GGGCAGGCCCCCCCCCCG—AGGAGGGAGGCT 3′	−
	G6C	313–344 (diG)	5′ GGGCAGGCCCCCCGTCCGCGAGGAGGGAGGCT 3′	−
	G56C	313–344 (diG)	5′ GGGCAGGCCCCCCGTCCG—AGGAGGGAGGCT 3′	−
RT-PCR	DM4	206–223 (satC)	5′ GGGACCAAAAACGGCGGC 3′	+
	GM4	206–223 (diG)	5′ TGGCAGCACTGTCTAGCT 3′	+
	T17 adapter		5′ CCACTCGAGTCGACATCGA(T)17 3′
	Adapter		5′ CCACTCGAGTCGACATCGA 3′
	(dT)18		5′ TTTTTTTTTTTTTTTTTT 3′
	284	284–301 (satC)	5′ ATCCCGAAAGGGTGGGCT 3′	−
RNA gel blot analysis	293	274–293 (satC)	5′ CTTTCGGGATTTTAGTGGTT 3′	−
		260–279 (diG)
CP-RNA binding	T7CG3′	292–301 (satC)	5′ GTAATACGACTCACTATAGGGTGGGCT 3′	+
	CG3′	344–356 (satC)	5′ GGGCAGGCCCCCC 3′	−

^aUnderlined, boldface sequences are inserted or mutated sequences. —, deletion of the nucleotides present in the wt diG sequence in G5C and G56C. 

^bPolarity refers to homology (+) or complementarity (−) with plus strands of subviral RNAs.