Extended Data Fig. 9 |. PPA treatment does not alter cardiac and respiratory rates in non-injured control mice but increases the high amplitude contraction frequency of cervical lymphatic vessels (CLV).

a-e, Group means were compared by student t-test (unpaired, two tailed). a, Heart rate (n = 9 mice; 4 control and 5 PPA, p = 0.4671). b, Respiration rate (n = 9 mice; 4 control and 5 PPA, p = 0.233). c, Mean arterial pressure (7 mice per group, p = 0.026). d, Cerebral blood flow (9 mice per group, p = 0.291). e, Intracranial pressure (8 mice per group, p = 0.0048). f, C57Bl6 mice implanted with cisterna magna cannula were injected with FITC dextran (10 μL) and recorded for contraction frequency (20–40 min post-injection). g, Contraction profile (representative segments, length 2 min) of CLV recorded in control (b) and with PPA administration (c) both under 2.5% isoflurane. h, High amplitude contractions (> 1.5-fold change in diameter) were quantified and shown as a box blot. Data (n = 17 mice, 8 control and 9 PPA mice, 1–2 representative recordings/mice), were compared using a student t-test (unpaired, two-tailed, F8,7 = 1.626, p = 0.0034). Data shown as box and whisker plots with the lower and upper quartile (box limits), median and min-max values (a–e, h) and line graph (g); the dots are biological replicates/mice. Scale: 500 μm.