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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Biol Psychiatry. 2023 Oct 29;95(8):785–799. doi: 10.1016/j.biopsych.2023.10.016

Figure 5. LS neurons modulated by TGOT and AVP are somatostatinergic, and more engaged by social-fear extinction in Magel2KO mice than in WT controls.

Figure 5.

(A) Infusion of Cadaverine dye in the patch pipette labeled type-1 cells counterstained with somatostatin (SST) antibodies. Scale=25μm.

(B) Proportion of type-1 cells (N=16) with SST marker. Scale=200μm.

(C) Binding specificity of the fluorescent peptide: 50 μM d[Lys(Alexa-647)8]VP without (total binding) or with 100 μM Manning compound (non-specific). OXTR binding sites marked with 10 μM d[Lys(Alexa-647)8]VP when AVPR were saturated with 5 μM of the competitive ligand Manning compound. AVPR binding sites marked with 50 μM d[Lys(Alexa-647)8]VP when OXTR were saturated with 5 μM of the competitive ligand TGOT.

(D) AVPR binding counter-stained with SST antibodies in dLS. Scale=25μm.

(E) A minority of SST-neurons in dLS (N=203) co-express AVPR binding sites.

(F) OXTR binding counter-stained with SST antibodies in dLS. Scale=25μm.

(G) A majority of SST-neurons in dLS (N=265) co-express OXTR binding sites.

(H) Co-staining with c-Fos and SST antibodies in dLS of Magel2KO mice and WT-controls sacrificed 1h after social-fear extinction.

(I) Proportion of SST-neurons co-labeled with c-Fos in LS. Data are means±SEM in N=6 mice/group. Mann-Whitney test p<0.05.