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The ORF 3 protein is associated with rNV VLPs. Recombinant rNV VLPs were purified from supernatants of Sf9 cells infected with the ORF 2 (rNV 2 VLPs) or the ORFs 2+3 (rNV 2+3 VLPs) baculovirus recombinants. VLPs sequentially purified over both CsCl and sucrose gradients were analyzed by SDS-PAGE and Western blotting using the rabbit ORF 3 C-terminal peptide antiserum (1:500).
