FIG. 7.

Western blot analysis of DI2/3 VLPs using the ORF 3 C-terminal peptide antiserum. VLPs were diluted to a loading concentration of 1 μg and serially diluted twofold for separation on an SDS-polyacrylamide gel. Western blot analysis was performed using the rabbit ORF 3 C-terminal peptide antiserum (1:500). Lanes: 1, 1 μg; 2, 0.5 μg; 3, 0.25 μg; 4, 0.125 μg; 5, 0.0625 μg; 6, 0.03125 μg; 7, 0.015625 μg. The DI2/3 particles were as pure as previously described VLP preparations (3).