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The ORF 3 protein comigrates with the ORF 2 protein in VLPs in sucrose gradients. Western blot analysis of the sucrose gradient fractions from the DI2/3 VLP purification is shown. Proteins were detected using the rabbit ORF 3 C-terminal peptide antiserum (A) or the ORF 2 MAb 3912 (B). Fractions numbers and gradient orientation are indicated. Each gel contained a VLP control, 2+3VLPs (left lanes).
