Table 2.

Comparison of specific protocols for scRNA-seq

Kit	scRNA-seq method	Genome coverage (%)	Advantages	Disadvantages
Sigma-Aldrich	DOP-PCR [8]	39	Low genome coverage	Highly suited for evaluating CNVs with large bin sizes (1 million bases) on a broad genomic scale
Qiagen	MDA [82]	84	High genome coverage	Sequence-dependent bias, overamplification in certain genomic regions, and underamplification in other regions. Normalization becomes impossible, and CNV determination becomes less precise
Yikon	MALBAC [82]	72	CNV may be determined by doing signal normalization for noise reduction	Sequence-dependent bias, a high false positive rate for SNV detection, under-amplified regions of the genome are sometimes lost during amplification and cannot be accessed owing to the reproducible sequence-dependent bias