FIG. 1.

TCR VB families in PBL and TIL. PBL were gated on CD4⁺ or CD8⁺ T cells and tested for expression of individual TCR VB chains by flow cytometry. No major TCR VB expansion could be detected in PBL. In contrast, the majority of TIL (>98% CD4⁺) stained positive for the TCR VB16. +, detection of monoclonal TCR VB chains as listed in Table 2.