TABLE 2.
Deduced TCR amino acid sequencesa
| T-cell population | TCR chain | Variable region sequence | CDR3 sequence | Sequence of joining region |
|---|---|---|---|---|
| PBL-VA CD8+ | VA2 | DSQPSDSATYLCA | VSG | NDMRFGAG (AJ43) |
| VA3* | ADTASYFCA | SRGGD | NTDKLIFGTG (AJ34) | |
| VA4 | TLRDAAVYYCI | LSFAS | AGGTSYGKLTFGQG (AJ52) | |
| VA9* | FAQEEDSAMYYCA | YGNNRLAFGKG (AJ7) | ||
| VA16 | SALVSDSALYFCA | VRHRI | DTGRRALTFGSG (AJ5) | |
| VA21 | QPGDSAVYFCA | ASES | GTSYGKLTFGQG (AJ52) | |
| VA23 | ASQPGDSATYLCA | VRYLL | SSGSARQLTFGSG (AJ22) | |
| VA25 | STYLCA | VGPS | SSGSARQLTFGSG (AJ22) | |
| PBL-VB CD8+ | VB2 | SAHPEDSSFYIC | SARGGGSG | NQPQHFGDG (BJ1-5) |
| VB3 | SASTNQTSMYLCA | SSLGVG | YEQYFGPG (BJ2-7) | |
| VB9 | LELGDSAVYFCA | SSQLWTSGAPP | STDTQYFGPG (BJ2-3) | |
| CD4+ TIL-VA | VA2 | FNTSCGTDYLCA | MSS | DRGSTLGRLYFGRG (AJ18) |
| VA3* | ADTASYFCA | SRGGD | NTDKLIFGTG (AJ34) | |
| VA6 | ASQLGDSAMYFCA | MREGW | AAGNKLTFGGG (AJ17) | |
| VA9* | FAQEEDSAMYYCA | YGNNRLAFGKG (AJ7) | ||
| VA10 (a) | AAQPGDTGLYLCA | GG | SDGQKLLFARG (AJ16) | |
| VA10 (b) | AAQPGDTGLYLCA | GGP | MDSSYKLIFGSG (AJ12) | |
| VA21 | PSQPGDSAVYFCA | ASGG | KLVFGKG (AJ57) | |
| VA26 | IYLCA | GG | SDGQKLLFARG (AJ16) | |
| CD4+ TIL-VB16 | VB16 | PAELDSGVYFCA | SSRGTSGTLK | QYFGPG (BJ2-7) |
| TIL VB16 sorted | VA10 | AAQPGDTGLYLCA | GGP | MDSSYKLIFGSG (AJ12) |
| VA21 | PSQPGDSAVYFCA | ASGG | KLVFGKG (AJ57) | |
| VA22 | AVYFCA | LS | YGCCRLAFGKG (AJ7) |
Monoclonal TCRs in CD8+ or CD4+ T-cell populations were identified by DNA sequence analysis as described previously (18). Exclusively the CDR3 region and the adjacent variable and joining TCR segments are listed. Note that the CD4+ VB16+ population represents 96% of all T cells in TIL (Fig. 1). Sorting of these VB16+ T cells yields a TCR VB16+ VA10+ VA21+ T-cell line. Note that in unsorted TIL, two TCR VA10 chains could be identified, but only one of these was present in the VB16-sorted T-cell population [TCR VA10(b)]. *, identical TCR sequences in PBL and TIL. TCR VA chains combined with VB16 exhibit a common CDR3 motif: TCR VA10 and TCR VA21 share glycine residues (bold), and TCR VA21 shares a serine residue (underlined) with VA22.