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. 2024 Apr 18;43(13):2552–2581. doi: 10.1038/s44318-024-00094-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV2 — (A, B) Transcript level by RNA seq (left) (n = 4, healthy donors used to obtain the pNK) and protein expression (n = 3 healthy donors) via flow cytometry (right) of (A) NFAT1 (NFATc2) and (B) NFAT2 (NFAT c1), respectively. The P value was calculated using a two-tailed t test, and the number of repeats is indicated within the graph presented as means ± SEM. (C) The transcript levels of TOX and TOX2 in anergic vs responsive cells (n = 4, where n is the number of healthy donors used to obtain the pNK cells). P values were calculated using a two-tailed t test with pairing and are indicated within the graph; NC normalized counts. (D) Purified anergic and responsive NK cells were lysed and subjected to western blot analysis with DGKα antibody (n = 6, where n is the number of healthy donors used to obtain the pNK cells). P values were calculated using a two-tailed t test with pairing, and are indicated within the graph presented as means ± SEM. Source data are available online for this figure.