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. 2024 Apr 18;43(13):2552–2581. doi: 10.1038/s44318-024-00094-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV3 — The gating strategy employed for the in vivo experiment. (A) NK cells were distinguished from the target PANC-1 cells according to FSC and SSC. CD45 (antibody specifically recognizing human (h)CD45) expression was used to further distinguish the pNK from the tumor and any other murine cells. Pseudocolor presentation is shown to identify the NK cells, which are CD45⁺ (95.9%) referred as tumor infiltrating NK cells (TINK). (B) Flow cytometry analysis of intracellular staining was performed to measure pNK expression on cells from the mice with tumor-only (no NK administration) control and distinguished based on CD45 expression. The NK cells were distinguished from the target cells based on their volume and density (FSC and SSC) and were re-gated to CD45⁺ subsets. DGKα and Egr2 expressions were measured on the CD45⁺ gated population. Histogram offsets and MFI were used for graphical presentation. Graphs show fluorescence intensity. The Y axis indicates relative cell number, and the X axis indicates the MFI. (C) Representative histograms and contour plots including outliers for the data in Fig. 3C–F.