Table 1.

DNA and RNA oligos for human EndoV nuclease activity assay (Related to Figs. 1–5 and Figures S1–S3).

Nuclease activity assay of human EndoV for inosine
Name	Sequence
ssDNA containing deoxyinosine	5'-CTGTATGAT	G	dI	rA	G	A	TGCTGAC-3'
ssDNA containing AP site	5'-CTGTATGAT	G	AP	rA	G	A	TGCTGAC-3'
ssDNA containing 6-methyladenine	5'-CTGTATGAT	G	Me-A	rA	G	A	TGCTGAC -3'
ssDNA containing xanthine	5'-CTGTATGAT	G	X	rA	G	A	TGCTGAC-3'

Nuclease activity assay of human EndoV for ribo-adenine
Name	Sequence
dI next to ribo-A	5'-CTGTATGAT	G	dI	rA	G	A	TGCTGAC -3'
dI next to deoxy-A	5'-CTGTATGAT	G	dI	dA	G	A	TGCTGAC -3'
dI next to 2'-O-Me-A	5'-CTGTATGAT	G	dI	O-Me-A	G	A	TGCTGAC -3'
dI next to 2'-F-A	5'-CTGTATGAT	G	dI	F-A	G	A	TGCTGAC -3'

Nuclease activity assay of human EndoV for nucleotide located -1 of cleavage site
Name	Sequence
dI next to ribo-A	5'-CTGTATGAT	G	dI	rA	G	A	TGCTGAC -3'
dI next to ribo-C	5'-CTGTATGAT	G	dI	rC	G	A	TGCTGAC -3'
dI next to ribo-G	5'-CTGTATGAT	G	dI	rG	G	A	TGCTGAC -3'
dI next to riboU	5'-CTGTATGAT	G	dI	rU	G	A	TGCTGAC -3'

Nuclease activity assay of human EndoV for nucleotide located + 1 cleavage site
Name	Sequence
dI two next to ribo-A	5'-CTGTATGAT	G	dI	rA	rA	A	TGCTGAC-3'
dI two next to ribo-C	5'-CTGTATGAT	G	dI	rA	rC	A	TGCTGAC-3'
dI two next to ribo-G	5'-CTGTATGAT	G	dI	rA	rG	A	TGCTGAC-3'
dI two next to ribo-U	5'-CTGTATGAT	G	dI	rA	rU	A	TGCTGAC-3'

Nuclease activity assay of human EndoV for nucleotide located + 2 of cleavage site
Name	Sequence
dI third next to ribo-A	5'-CTGTATGAT	G	dI	rA	rA	rA	TGCTGAC-3'
dI third next to ribo-C	5'-CTGTATGAT	G	dI	rA	rA	rC	TGCTGAC-3'
dI third next to ribo-G	5'-CTGTATGAT	G	dI	rA	rA	rG	TGCTGAC-3'
dI third next to ribo-U	5'-CTGTATGAT	G	dI	rA	rA	rU	TGCTGAC-3'

Nuclease activity assay of human EndoV for nucleotide located -3 of cleavage site
Name	Sequence
dI ahead of ribo-A	5'-CTGTATGAT	rA	dI	A	G	A	TGCTGAC-3'
dI ahead of ribo-C	5'-CTGTATGAT	rC	dI	A	G	A	TGCTGAC-3'
dI ahead of ribo-G	5'-CTGTATGAT	rG	dI	A	G	A	TGCTGAC-3'
dI ahead of ribo-U	5'-CTGTATGAT	rU	dI	A	G	A	TGCTGAC-3'

Nuclease activity assay of human EndoV for ribo-adenine
Name	Sequence
dl next to rA dA dA	5'-CTGTATGAT	G	dI	rA	dA	dA	TGCTGAC-3'
dl next to rA rA dA	5'-CTGTATGAT	G	dI	rA	rA	dA	TGCTGAC-3'
dl next to rA dA dA	5'-CTGTATGAT	G	dI	rA	rA	rA	TGCTGAC-3'

Nuclease activity assay of human EndoV for poly-A
Name	Sequence
Poly-A	5'-rArArArArArArArArArArArArArArArArArArArArA- 3'
i-PolyA	5'-rArArArArArArArArArArIrArArArArArArArArArA-3'