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. 2024 May 20;43(13):2759–2788. doi: 10.1038/s44318-024-00120-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A) Immunoblot of HEK293T cells transfected with shCtrl or two independent HAX1 shRNAs. (B, C) Control and HAX1 knockdown (shHAX1) HEK293T cells were stained for LSM14A and DAPI. Representative images are shown in (B). The number of P-bodies within each cell is plotted in (C) (n = 50). Scale bar, 5 μm. Error bars indicate SEM. ****P < 0.0001 (Student’s t test). (D) The expression of HAX1, 4E-T, DCP1A, DDX6, and LSM14A in HAX1 KO HEK293T cells was detected by immunoblotting. (E, F) Control and HAX1 KO HEK293T cells were exposed to sodium arsenite (2 h) and immunolabeled for TIA1 and G3BP2 (E). Scale bar, 5 μm. The number of stress granules within each cell is plotted in (F) (n = 50). Statistical Error bars indicate SEM. Ns: no significance (two-way ANOVA). (G) S2 and P2 fractions from HEK293T cells treated with oligomycin for the indicated times were analyzed by immunoblotting. (H) S2 and P2 fractions from HAX1 KO HEK293T cells transfected with HAX1^WT or HAX1^K131R along with sgCrtl or sgTRIM23 were analyzed by immunoblotting. (I, J) S2 and P2 fractions from control and HAX1-knockdown (shHAX1) HEK293T cells with or without oligomycin treatment (1 h) (I) or TRIM23 overexpression (J) were analyzed by immunoblotting.