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. 2024 May 20;43(13):2759–2788. doi: 10.1038/s44318-024-00120-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Immunoblot of indicated cells exposed to puromycin (10 μg/ml, 30 min). HEK293T cells transfected with vector or TRIM23 (A), control and TRIM23-overexpressing (TRIM23 o/v) HCT116 cells (B), control and TRIM23 KO HEK293T cells treated with or without oligomycin (1 h) (C), control and TRIM23 KO RKO cells treated with or without oligomycin (1 h) (D), control and HAX1 KO HEK293T cells treated with or without oligomycin (1 h) (E), HAX1 KO HEK293T cells transfected with vector, HAX1^WT or HAX1^K131R (F), or HAX1 KO HEK293T cells transfected with Flag-HAX1^ΔIDR2 or Flag-HAX1^ΔIDR2-IDR^FUS (G), or with Flag-HAX1^K131R or Flag-HAX1^K131R-IDR^FUS (H) were analyzed for newly synthesized proteins containing puromycin. Source data are available online for this figure.