Fig. 2. Concomitant draining lymph node irradiation does not affect the tumor response to radiotherapy alone.

A B16F10-Luc tumor-bearing mice received tumor IR, with or without concomitant DLN IR. “Sham IR” group (black) was sham-irradiated, “TM IR” group (blue) received tumor IR and “TM + C-DLN IR” group (orange) received DLN IR concomitantly to tumor IR. Tumor growth was followed over 50 days. B–E Treatment response represented by Kaplan–Meier survival analysis (B and E), individual tumor growth curves (C) and a waterfall plot derived from the mRECIST analysis (D). Time to reach 1000 mm³ was used as the endpoint for Kaplan–Meier analysis. Each line in C and each bar in D represents an individual mouse. Parameters derived from the mRECIST analysis in D and E are described in the Methods section. mCR, complete response; mPR, partial response; mSD, stable disease; mPD, progressive disease. Number of mice in each group is indicated in the corresponding graph title in C. Logrank test (Mantel–Cox) was used to compare the survival curves; corresponding p values are displayed in E. All p values are displayed, with *, ** and *** indicating p < 0.05, p < 0.01 and p < 0.001, respectively. Source data are provided as a Source Data file. Figure A, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.