Skip to main content
. 2024 May 27;27(6):110131. doi: 10.1016/j.isci.2024.110131

Figure 6.

Figure 6

PICALM KO cells exhibit low PD-1 levels and a modulated differentiation into TEM-like cells

SupT1 NTC and PICALM KO cells were infected with HIV Nef-CRIMZs virus and were harvested 48 h post-infection.

(A) Dot plot representation of analyzed populations in (B and C). (B) Representative histograms of PD-1 expression in uninfected, latently- and productively-infected cells. (C) Bar graph quantification of data shown in (B) demonstrating that PD-1 levels in PICALM KO cells are modulated and are differently expressed in uninfected, productive and latently infected cells.

(D and E) CD45RA, CCR7 and CD27 were measured to determine that PICALM KO cells show a decreased TEM-like phenotype. Gating strategy was performed as mentioned in Figure 5C, following the gating of viable cells, cells were gated on CD45Ra−, then on CCR7−CD27− as shown in panel (D). (E) Graphical representation of data in (D) illustrating the frequency of TEM-like cells.

(F) Quantification of PD-1 expression (MFI) in TEM-like cells, showing that PICALM KO leads to lower levels of PD-1. Relative values (%) were calculated by setting NTC values to 100%. Graphs show mean ± SEM. ANOVA one-way, with Tukey post-test. ∗p < 0.05; ∗∗p < 0.001; ∗∗∗p < 0.0001.