Figure 2.

Effect of NP on the expression of tyrosine hydroxylase. Normal and PD mice were treated with ZnO, CoFe₂O₄, or their combination. TH expression was assayed using western blot analysis. A quantitative assessment of bands was done using ImageJ. Expression levels of treated samples and controls were normalized to their respective GAPDH, and then fold expression was determined relative to the control. A: Normal, B: Normal+ ZnO NP, Normal+ CoFe₂O₄ NP, D: Normal+ dual NP, E: PD, F: PD+ ZnO NP, F: PD+CoFe₂O₄ NP, PD+ dual NP. The data presented is the mean ± SD of determinations from different experiments. Asterisks on bars represent significance relative to the control, and those drawn upwards represent inter-categorical statistical significance. (*), (**), (***), and (****) correspond to P < 0.05, 0.01, and 0.001; respectively.