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. 2024 Jul 1;12(7):e009410. doi: 10.1136/jitc-2024-009410

Figure 3.

Figure 3

Inhibition of NKG2A− NK cells by IFNγ-pretreated A375 cells deficient in classical MHC-I molecules. (A, B) Expression of HLA-B and HLA-C on A375 cells treated or not with IFNγ for 12, 24 and 48 hours (n=6). (C) Degranulation of NKG2A− NK after 4 hours of co-culture with WT, HLA-B KO and HLA-C KO A375 cells pretreated or not with IFNγ 12 hours before the co-culture (n=6). (D) Degranulation of bulk NK cells and (E) NKG2A− and NKG2A+ NK cells after 4 hours of co-culture with WT and HLA-BC dKO A375 cells pretreated or not with IFNγ 12 hours before the co-culture. (n=6). (F) Expression of KIRs on 2 days IL-2-cultured NKG2A− or NKG2A+ NK cell subsets (n=5–9). Statistical analysis was performed by one-way ANOVA (multiple comparisons) test (A–E) and two-tailed Student’s t-test (F), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ANOVA, analysis of variance; KO, knockout; NK, natural killer; WT, wild-type.