Fig. 2.

Using the CellPopAge Clock for the detection of anti-ageing drugs. A Schematic illustrating the experimental set-up conducted in P9 to P20 HDFs and HMFs, passaged weekly and continuously treated with either rapamycin, trametinib, torin2, or dactolisib/BEZ235, represented as a pill. Control cells were treated with vehicle, either DMSO or ethanol. B The CellPopAge Clock predictions of human dermal fibroblasts (HDF) and C human mammary fibroblasts (HMF). Represented is predicted-actual passage for passages 16, 18, and 20, showing deceleration of the CellPopAge Clock upon treatment with anti-ageing drugs rapamycin (5 nM), trametinib (0.1 nM), torin2 (5 nM), and dactolisib/BEZ235 (10 nM) and non-treated control samples (black dots)