Fig. 4.

Drugs that decelerate the CellPopAge Clock extend lifespan in vivo. A Lifespan analysis on w^Dah background wild-type flies fed with holidic food containing different concentration of rapamycin or ethanol as solvent control. For each condition, 150 flies were used. B Lifespan analysis on w^Dah background wild-type flies fed with holidic food containing different concentration of dactolisib/BEZ235 or DMSO as solvent control. For each condition, 150 flies were used. C Lifespan analysis on w^Dah background wild-type flies fed with holidic food containing different concentration of torin2 or DMSO as solvent control. For each condition, 150 flies were used. D Schematic representation of our proposed screening platform which combines our novel CellPopAge Clock and other ageing biomarkers in vitro primary human cell, together with in vivo Drosophila lifespan experiments, for a detailed and robust capture of anti-ageing drug potential