TABLE 2.
Matcha catechin components with MIC and aggregation activity
| ID | Catechin | Description (molecular weight/functional group) | Content (%)a | MIC (µM)b | Aggregation activityc |
|---|---|---|---|---|---|
| M-1 | Catechin | 290.26 | 0.3 | >500 | 69.7% |
| M-2 | Epicatechin | 290.26/Epi (+) | 3.5 | >500 | 70.2% |
| M-3 | Gallocatechin | 306.27/Gallo (+) | 1.2 | 500 | 68.1% |
| M-4 | Epigallocatechin | 306.27/Epi (+), Gallo (+) | 18.5 | 250 | 68.3% |
| M-5 | Epicatechin-3-O-gallate | 456.40/Epi (+), 3-O-gallate (+) | 8.6 | >500 | 89.0% |
| M-6 | Gallocatechin-3-O-gallate | 456.40/Gallo (+), 3-O-gallate (+) | 0.7 | 250 | NT |
| M-7 | Epigallocatechin-3-O-gallate | 458.37/Epi (+), Gallo (+), 3-O-gallate (+) | 57.3 | 500 | 72.1% |
| M-8 | Epigallocatechin-3-O-(3"O-methyl)-gallate | 472.40/Epi (+), Gallo (+), 3-O-(3"-O-methyl)-gallate (+) | ND | 500 | NT |
| M-9 | 1,4,6-tri-O-galloyl-glucose | 636.47/Gallo (+++) | ND | 500 | NT |
| |||||
a
Contents of catechins (%) in matcha were analyzed using micellar electrokinetic chromatography as described in reference 37.
b
MIC (µM) was determined by microdilution.
c
Aggregation activity (%) was calculated as 100 × [the value of A600 2 h after addition of each catechin/the value of OD600 2 h after addition of the vehicle control (DMSO)].