Fig 5.

Altered pigment production does not impact the virulence of J2315 or K56-2 in a CGD mouse lung infection. Mice were infected intratracheally with ∼10³ CFU/mouse and the bacterial load in lung homogenates of mice infected with the wild-type or indicated isogenic mutants of (A) J2315 or (B) K6-2 was assessed. (C) A parallel group of mice were monitored for survival. For colonization experiments, mice were euthanized at indicated time points post-infection, and lungs were aseptically collected and homogenized in 1 mL PBS. All samples were plated for viable CFU on Pseudomonas isolation agar. Each dot represents the CFU from a single mouse. Error bars represent standard deviation (SD). Results were compiled from two independent experiments. For survival experiments, animals were monitored for 12 days post-infection. Results are represented by Kaplan-Meier survival curves and were analyzed by log rank test. Results were compiled from two independent experiments, n = 8/strain. Unlabeled comparisons were not statistically significant.