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Primers used for the C2V3 region of HIV-1/O amplification and sequencing
| PCR step | Sequence (5′−3′) | Fragment size (bp) |
|---|---|---|
| External | GGCTTTGMTAAYCCCATGTTTGA | 1,533 |
| CCCATAGTGCTTCCTGCTGC | ||
| Nested | ATTCCAATACACTATTGTGCTCCA | 518 |
| AAAGAATTCTCCATGACAGTTAAA | ||
| Sequencing | ATTCCAATACACTATTGTGCTCCA | 518 |
| AAAGAATTCTCCATGACAGTTAAA |
