TABLE 2.
Description and summary of the bacterial strains used in this study.
| Strain | Description | Ref |
|---|---|---|
| Pseudomonas aeruginosa UCBPP-PA14 | Pseudomonas aeruginosa UCBPP-PA14 | (81) |
| Pseudomonas aeruginosa UCBPP-PA14 chiC::MAR2xT7 D7 (PA14NR: 26353) | Pseudomonas aeruginosa UCBPP-PA14 transposon mutant, chiC::MAR2xT7. Features a transposon in chiC, resulting in the inactivation of the chitinase protein | (53) |
| Pseudomonas aeruginosa UCBPP-PA14 chiC::MAR2xT7 D11 (PA14NR: 42166) | Pseudomonas aeruginosa UCBPP-PA14 transposon mutant, chiC::MAR2xT7. Features a transposon in chiC, resulting in the inactivation of the chitinase protein | (53) |
| Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa PAO1 | (82) |
| Pseudomonas aeruginosa PAO1 strain PW4887 (chiC::ISphoA/hah) | Pseudomonas aeruginosa PAO1 transposon mutant, chiC::ISphoA/hah. Features a transposon in chiC, resulting in the inactivation of the chitinase protein | (82) |
| Escherichia coli BL21 Star (DE3) pNIC-CH ChiC (PA2300) | Strain for expression and purification of ChiC | This study |
| E. coli BL21 Star (DE3) pNIC-CH ChiC | Strain for expression and purification of ChiC with His-tag | This study |
| E. coli BL21 Star (DE3) pNIC-CH ChiCE143Q | Strain for expression and purification of ChiC with His-tag and mutated active site | This study |
| Vibrio natriegens Vmax X2 pET28b SmChiC | Strain for expression and purification of SmChiC | This study |