. 2024 Jun 11;12(7):e03524-23. doi: 10.1128/spectrum.03524-23

TABLE 1.

List of species-specific primers for monitoring the probiotic preparation with quantitative real-time PCR and related working features

Species	Primers	Primer sequence (5′–3′)	Target gene	Product size (bp)	T annealing (°C)	Reference
Lp. plantarum	Lp-F	AAAATCATGCGTGCGGGTAC	pyrG	261	55	(27)
Lp. plantarum	Lp-R	ATGTTGCGTTGGCTTCGTCT	pyrG	261	55	(27)
Ls. reuteri	Lreu-1	CAGACAATCTTTGATTGTTTAG	16S-23S spacer region	305	60	(27)
Ls. reuteri	Lreu-4	GCTTGTTGGTTTGGGCTCTTC	16S-23S spacer region	305	60	(27)
Lc. paracasei	PC2a	GGATTGGGTTTTGCGTGATGGTCGC	mutL	261	68	(27)
Lc. paracasei	CPRrev	TGCATTTCCCCGCTTTCATGACT	mutL	261	68	(27)
B. megaterium	BmphaC015F	CGTGCAAGAGTGGGAAAAAT	phaC	900	64	(28)
B. megaterium	BmphaC931R	TCGCAATATGATCACGGCTA	phaC	900	64	(28)
B. pumilus	rpoB_F	ATCGAAACGCCTGAAGGTCCAAACAT	rpoB	1,200	52	(29)
B. pumilus	rpoB_R	ACACCCTTGTTACCGTGACGACC	rpoB	1,200	52	(29)