Real-time PCR quantification of cDNA generated from HSV-1 RNA in dorsal root ganglia of mice infected with NS, NS-gEnull (labeled null), or NS-gE380 (labeled 380) virus and mock-infected controls. RNA was quantified for an immediate-early gene, ICP27, an early gene, US9, and a late gene, gC. The number of viral transcripts was calculated in comparison with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a control transcript expressed constitutively in cells. Results are the means ± SEM for ganglia from three mice in each group.