TABLE 1.

Primers and probes used for PCR amplification of HSV-1 and genomic DNA or cDNA

Primer	Location	Size (bp)	Sequence
gC (UL44)	5′	19	GATGCCGGTTTCGGAATTC
	3′	22	CCCATGGAGTAACGCCATATCT
	Probe	21	ACCCGCATGGAGTTCCGCCTC
US9	5′	17	AGGCGGCCAACGACTTC
	3′	21	TCGACGCCTTAATACCGACTG
	Probe	19	TCGTACGCATGGGCCGCCA
ICP27 (UL54)	5′	21	CGCCAAGAAAATTTCATCGAG
	3′	19	ACATCTTGCACCACGCCAG
	Probe	20	CTGGCCTCCGCCGACGAGAC
GAPDHa (control gene)	5′	23	CAAGGTCATCCATGACAACTTTG
	3′	20	GGCCATCCACAGTCTTCTGG
	Probe	25	ACCACAGTCCATGCCATCACTGCCA

^aGAPDH, glyceraldehyde-3-phosphate dehydrogenase.